5YUN
Crystal structure of SSB complexed with myc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2017-11-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 31 |
| Unit cell lengths | 60.110, 60.110, 130.868 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.670 |
| R-factor | 0.20986 |
| Rwork | 0.207 |
| R-free | 0.25648 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyg |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.617 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.780 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Number of reflections | 14947 | |
| <I/σ(I)> | 17.29 | |
| Completeness [%] | 99.5 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 25%PEG4000, 100mM MES Sodium Salt, 200mM Magnesium Chloride |
