5YUF

Crystal Structure of PML RING tetramer

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-12
Detector	MAR CCD 130 mm
Wavelength(s)	1.2824
Spacegroup name	P 21 21 21
Unit cell lengths	38.510, 84.690, 86.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.825 - 1.600
R-factor	0.2035
Rwork	0.203
R-free	0.21680
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.954
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	CRANK2
Refinement software	PHENIX ((1.11.1_2575: v1.0))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.400	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.140	1.330
Number of reflections	37754
<I/σ(I)>	8
Completeness [%]	99.4
Redundancy	2.4
CC(1/2)	0.996	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Ammonium Sulfate