5YTV
Crystal structure of YB1 cold-shock domain in complex with UCAUCU
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 62 |
Unit cell lengths | 66.275, 66.275, 35.195 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.003 - 1.700 |
R-factor | 0.1992 |
Rwork | 0.196 |
R-free | 0.22760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pf5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.071 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.003 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 9693 | |
<I/σ(I)> | 30 | |
Completeness [%] | 100.0 | |
Redundancy | 16.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 290 | 30 % (v/v) 2-methyl-2,4-pentanediol, 0.1 M sodium acetate, pH 4.5, 25 % (w/v) PEG 1500 |