5YTM
C135A mutant of copper-containing nitrite reductase from Geobacillus thermodenitrificans determined by in-ouse source
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-02-13 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 115.078, 115.078, 84.043 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.507 - 1.500 |
| R-factor | 0.1186 |
| Rwork | 0.117 |
| R-free | 0.15300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.851 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.074 | 0.490 |
| Number of reflections | 65738 | 3274 |
| <I/σ(I)> | 22.8 | |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 1.8 | |
| CC(1/2) | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M acetate buffer pH 4.5, 5.0% PEG 4000 |
