5YT6
Crystal structure of TAX1BP1 UBZ2 in complex with mono-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97846 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.709, 72.938, 119.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.894 - 1.501 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bmj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.125 | 0.097 | 0.465 |
| Rmeas | 0.137 | 0.107 | 0.538 |
| Rpim | 0.056 | 0.044 | 0.263 |
| Total number of observations | 419962 | ||
| Number of reflections | 68493 | 3693 | 3140 |
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 99.2 | 99.1 | 92 |
| Redundancy | 6.1 | 5.8 | 3.9 |
| CC(1/2) | 0.989 | 0.763 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | Ammonium sulfate, BIS TRIS propane |
