5YT6

Crystal structure of TAX1BP1 UBZ2 in complex with mono-ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97846
Spacegroup name	P 21 21 21
Unit cell lengths	48.709, 72.938, 119.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.894 - 1.501
R-factor	0.195
Rwork	0.193
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bmj
RMSD bond length	0.006
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.125	0.097	0.465
Rmeas	0.137	0.107	0.538
Rpim	0.056	0.044	0.263
Total number of observations	419962
Number of reflections	68493	3693	3140
<I/σ(I)>	5.9
Completeness [%]	99.2	99.1	92
Redundancy	6.1	5.8	3.9
CC(1/2)		0.989	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Ammonium sulfate, BIS TRIS propane