5YSP
Pyrophosphate-dependent kinase in the ribokinase family complexed with a pyrophosphate analog and myo-inositol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.673, 63.009, 89.884 |
| Unit cell angles | 90.00, 105.05, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.19713 |
| Rwork | 0.196 |
| R-free | 0.21042 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vk4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.382 |
| Data scaling software | HKL-2000 (715) |
| Phasing software | MOLREP (11.2.08) |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 54308 | |
| <I/σ(I)> | 24.8 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | PEG 4000, ammonium sulfate, sodium acetate |
