5YSF
Crystal structure of beta-1,2-glucooligosaccharide binding protein in complex with sophoropentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.218, 125.988, 91.040 |
| Unit cell angles | 90.00, 100.45, 90.00 |
Refinement procedure
| Resolution | 44.760 - 1.900 |
| R-factor | 0.16823 |
| Rwork | 0.166 |
| R-free | 0.20315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yse |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.864 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.099 | 0.545 |
| Rpim | 0.059 | 0.323 |
| Number of reflections | 61562 | 2964 |
| <I/σ(I)> | 16.2 | 2.1 |
| Completeness [%] | 97.5 | 95.6 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.824 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 298 | 0.15M MES-NaOH, pH 5.3, 42% (v/v) MPD |
