5YSC
Crystal Structure of periplasmic Vitamin B12 binding protein BtuF of Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 55.877, 55.877, 279.163 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.680 - 1.673 |
| R-factor | 0.1992 |
| Rwork | 0.197 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n2z |
| RMSD bond length | 0.063 |
| RMSD bond angle | 3.033 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.680 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.071 | 0.462 |
| Number of reflections | 31146 | |
| <I/σ(I)> | 28.2 | 3.3 |
| Completeness [%] | 99.5 | 91 |
| Redundancy | 14.08 | 14.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.8M ammonium sulfate, 0.1M Tris (pH 8.0) were equilibrated against a 1.6M ammonium sulfate, 0.1M HEPES |
