5YSB

Crystal structure of beta-1,2-glucooligosaccharide binding protein in ligand-free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-28
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 32
Unit cell lengths	74.925, 74.925, 120.844
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.240 - 2.200
R-factor	0.19246
Rwork	0.190
R-free	0.24679
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uor
RMSD bond length	0.016
RMSD bond angle	1.667
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.097	0.571
Rpim	0.047	0.263
Number of reflections	38141	1912
<I/σ(I)>	21
Completeness [%]	98.8	100
Redundancy	5.5	5.7
CC(1/2)		0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.15M zinc acetate, 15% (w/v) PEG 3350