5YS1
Crystal structure of Multicopper Oxidase CueO G304K mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.254, 50.650, 66.231 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.490 |
| R-factor | 0.1468 |
| Rwork | 0.145 |
| R-free | 0.18010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n68 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.863 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.040 | 0.459 |
| Number of reflections | 65684 | |
| <I/σ(I)> | 26.9 | |
| Completeness [%] | 98.6 | 88.6 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.2M Sodium formate (pH 8.5), 20%(w/v) Polyethyleneglycerol 3,350 |
