5YS0
Crystal structure of the second StARkin domain of Lam2 in complex with ergosterol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.717, 70.315, 42.508 |
| Unit cell angles | 90.00, 103.14, 90.00 |
Refinement procedure
| Resolution | 32.709 - 2.601 |
| R-factor | 0.2436 |
| Rwork | 0.241 |
| R-free | 0.29350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yqj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.039 | 0.331 |
| Number of reflections | 16717 | 845 |
| <I/σ(I)> | 48.9 | 6.9 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1M Tris-HCl pH 8.0, 30% PEG8000. |
