5YRZ

Toxin-Antitoxin complex from Streptococcus pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	77.2
Detector technology	CCD
Collection date	2014-11-22
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	106.263, 116.541, 42.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.669 - 2.304
R-factor	0.2061
Rwork	0.204
R-free	0.23610
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.963
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	24090
<I/σ(I)>	30.2
Completeness [%]	99.7
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	277	0.1 M Tris, pH 8.5, 2.0 M Ammonium sulfate