5YRX
Crystal structure of a hypothetical protein Rv3716c from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-07-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 35.110, 183.750, 37.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.000 - 1.900 |
| R-factor | 0.17423 |
| Rwork | 0.172 |
| R-free | 0.21594 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ybx |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.282 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.250 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.227 |
| Rmeas | 0.086 | 0.244 |
| Rpim | 0.025 | 0.091 |
| Number of reflections | 9968 | 625 |
| <I/σ(I)> | 24.6 | 12.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.7 | 13.5 |
| CC(1/2) | 0.997 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.5 | 293 | 20mM Sodium phosphate pH 5.5 0.1M Cadmium chloride 0.1M Magnesium chloride 0.1M Nickel chloride 24% PEG 4000 |
