5YR3
Structure of sfYFP66BPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2017-06-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 51.713, 51.713, 178.977 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.432 - 1.901 |
| R-factor | 0.2486 |
| Rwork | 0.244 |
| R-free | 0.28960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qyo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.135 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.434 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 19926 | 3150 |
| <I/σ(I)> | 17.7 | 3.89 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 5.8 | 5.9 |
| CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289.15 | 15% PEG 3350, 0.1M malic acid, pH 5.0 |
