5YQR

Crystal structure of the PH-like domain of Lam6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-14
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	59.543, 60.103, 105.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.374 - 2.402
R-factor	0.2207
Rwork	0.218
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n9n
RMSD bond length	0.008
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.098	0.412
Number of reflections	15204	727
<I/σ(I)>	48.3	9.7
Completeness [%]	99.0	100
Redundancy	8.1	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.1M HEPES-HCl pH 7.0, 10% PEG8000, 0.1M Na3Citrate