5YQJ
Crystal structure of the first StARkin domain of Lam4
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.813, 54.950, 119.478 |
Unit cell angles | 90.00, 95.08, 90.00 |
Refinement procedure
Resolution | 29.752 - 1.500 |
R-factor | 0.1605 |
Rwork | 0.158 |
R-free | 0.21060 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.731 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.062 | 0.370 |
Number of reflections | 80061 | 3964 |
<I/σ(I)> | 31.6 | 4.8 |
Completeness [%] | 99.6 | 99 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 0.1 M Na-Citrate-HCl pH 5.0, 30% PEG 1500, 0.1 M Li2SO4 |