5YQ5
Crystal structure of human osteomodulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.640, 110.700, 122.150 |
Unit cell angles | 90.00, 106.97, 90.00 |
Refinement procedure
Resolution | 35.190 - 2.170 |
R-factor | 0.21837 |
Rwork | 0.217 |
R-free | 0.24526 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xku |
RMSD bond length | 0.015 |
RMSD bond angle | 1.736 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.190 | 2.280 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.084 | 0.644 |
Number of reflections | 101333 | 14545 |
<I/σ(I)> | 9.2 | 1.9 |
Completeness [%] | 99.2 | 97.9 |
Redundancy | 3.6 | 3.1 |
CC(1/2) | 0.997 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 200 mM ammonium phosphate dibasic 24 % PEG 3350 |