5YQ5
Crystal structure of human osteomodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.640, 110.700, 122.150 |
| Unit cell angles | 90.00, 106.97, 90.00 |
Refinement procedure
| Resolution | 35.190 - 2.170 |
| R-factor | 0.21837 |
| Rwork | 0.217 |
| R-free | 0.24526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xku |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.736 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.190 | 2.280 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.084 | 0.644 |
| Number of reflections | 101333 | 14545 |
| <I/σ(I)> | 9.2 | 1.9 |
| Completeness [%] | 99.2 | 97.9 |
| Redundancy | 3.6 | 3.1 |
| CC(1/2) | 0.997 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 200 mM ammonium phosphate dibasic 24 % PEG 3350 |
