5YQ4

Crystal structure of kelch domain of KLHL20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-28
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	49.957, 74.203, 155.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.580
R-factor	0.1646
Rwork	0.163
R-free	0.19140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	2.205
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.640
High resolution limit [Å]	1.580	3.400	1.580
Rmerge	0.049	0.028	0.236
Rmeas	0.052	0.029	0.251
Rpim	0.017	0.009	0.084
Total number of observations	384030
Number of reflections	40146	4221	3937
<I/σ(I)>	12.4
Completeness [%]	99.9	99.8	99.5
Redundancy	9.6	9.4	8.6
CC(1/2)		0.998	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	283	0.1 M Sodium Citrate, pH 5, 20% PEG 6000