5YPG

p62/SQSTM1 ZZ domain with Leu-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	173
Detector technology	CCD
Collection date	2017-05-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	28.534, 44.279, 68.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.000 - 2.199
R-factor	0.2414
Rwork	0.236
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.592
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall
Low resolution limit [Å]	27.000
High resolution limit [Å]	2.199
Number of reflections	4633
<I/σ(I)>	25.3
Completeness [%]	98.3
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	MgCl2, Sodium cacodylate