5YP8
p62/SQSTM1 ZZ domain with Arg-peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2015-10-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.981, 43.948, 66.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 1.448 |
| R-factor | 0.1626 |
| Rwork | 0.159 |
| R-free | 0.19420 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.086 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.810 |
| High resolution limit [Å] | 1.448 |
| Number of reflections | 15275 |
| <I/σ(I)> | 49.5 |
| Completeness [%] | 97.3 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 400, PEG 300, Tris, NaCl |
