5YOH
Mycobacterium Tuberculosis Methionine aminopeptidase type 1c (C105M mutant) in complex with Methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-05-25 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.332, 48.690, 56.151 |
Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
Resolution | 28.590 - 1.630 |
R-factor | 0.1683 |
Rwork | 0.166 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yj3 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.159 |
Data reduction software | DENZO (2.3.1) |
Data scaling software | SCALEPACK (2.2.0) |
Phasing software | HKL-2000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.590 | 3.700 | 1.680 |
High resolution limit [Å] | 1.620 | 3.490 | 1.620 |
Rmerge | 0.027 | 0.022 | 0.118 |
Rmeas | 0.038 | 0.031 | 0.166 |
Rpim | 0.026 | 0.022 | 0.117 |
Number of reflections | 32311 | 3114 | |
<I/σ(I)> | 59.8 | ||
Completeness [%] | 92.2 | 97.2 | 78.1 |
Redundancy | 1.9 | 1.9 | 1.9 |
CC(1/2) | 0.998 | 0.997 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Hepes, 27% Peg3350, 3% glycerol |