5YO8
Crystal structure of beta-C25/C30/C35-prene synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.350, 151.670, 53.840 |
| Unit cell angles | 90.00, 110.03, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.640 |
| R-factor | 0.20547 |
| Rwork | 0.204 |
| R-free | 0.22934 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.119 |
| Data reduction software | XDS |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.058 | 0.678 |
| Number of reflections | 92698 | 6015 |
| <I/σ(I)> | 11.3 | 2 |
| Completeness [%] | 94.4 | 87.7 |
| Redundancy | 3.24 | 3.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 293 | 15% PEG 3350, 0.1M BICINE-Na (pH 9.0), 0.2M Ammonium phosphate dibasic |
