5YO4
Crystal Structure of B562RIL with engineered disulfide bond K27C-A79C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-18 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 41.766, 51.287, 89.894 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.386 - 1.370 |
R-factor | 0.184 |
Rwork | 0.181 |
R-free | 0.21250 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.860 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.390 |
High resolution limit [Å] | 1.370 |
Number of reflections | 20635 |
<I/σ(I)> | 45.75 |
Completeness [%] | 99.0 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 3.2M NH4(SO4), 0.1M Bicine 2.44mM N-octanoylsucrose |