5YNO

Crystal structure of MERS-CoV nsp16/nsp10 complex bound to SAH and m7GpppA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-03
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9778
Spacegroup name	P 21 2 21
Unit cell lengths	67.193, 70.186, 119.353
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.536 - 1.960
R-factor	0.1944
Rwork	0.193
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r24
RMSD bond length	0.010
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.550
High resolution limit [Å]	1.960	1.960
Rmerge	0.064
Number of reflections	82216	4028
<I/σ(I)>	21.3	2.9
Completeness [%]	99.8	99.3
Redundancy	10.6	10.6
CC(1/2)	0.999	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	10% PEG 5000 ME, 5% Tascimate