5YNN
Crystal structure of MERS-CoV nsp16/nsp10complex bound to sinefungin and m7GpppG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 67.222, 70.045, 119.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.549 - 1.860 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r24 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.433 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.560 | |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.090 | |
| Number of reflections | 194100 | 4755 |
| <I/σ(I)> | 16.6 | 2.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 12 | 11.5 |
| CC(1/2) | 0.999 | 0.866 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 10% PEG 5000 ME, 5% Tascimate |
