5YMU
Functional and structural characterization of P[19] rotavirus VP8* interaction with histo-blood group antigens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2017-07-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97775 |
| Spacegroup name | H 3 |
| Unit cell lengths | 131.396, 131.396, 123.592 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.860 - 1.800 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gj6 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.796 |
| Number of reflections | 74097 |
| <I/σ(I)> | 21.895 |
| Completeness [%] | 100.0 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.1 M MES monohydrate pH 6.0, 25% w/v polyethylene glycol 4000 |
