5YLO

Structural of Pseudomonas aeruginosa PA4980

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 63 2 2
Unit cell lengths	124.958, 124.958, 111.468
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.820 - 2.390
R-factor	0.241
Rwork	0.238
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.981
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.822	2.480
High resolution limit [Å]	2.391	2.390
Number of reflections	20824
<I/σ(I)>	29.4375	3.15
Completeness [%]	99.8	98.2
Redundancy	26.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1M Tris/HCl pH 8.5, 3.0M NaCl