5YLG
Crystal Structure of LysM domain from pteris ryukyuensis chitinase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-06-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.839, 50.125, 92.362 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.180 - 1.480 |
| R-factor | 0.18568 |
| Rwork | 0.184 |
| R-free | 0.21974 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pxv |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.313 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 30690 | 12086 |
| <I/σ(I)> | 57.6 | 6.7 |
| Completeness [%] | 99.4 | 89.9 |
| Redundancy | 10.6 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 M Zinc acetate dihydrate, 25% PEG 3350, 20% glycerol |
