5YL8
The crystal structure of inactive dimeric peptidyl-tRNA hydrolase from Acinetobacter baumannii at 1.79 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.530, 98.490, 123.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.900 - 1.790 |
| R-factor | 0.1767 |
| Rwork | 0.175 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y98 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.087 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.900 | 1.818 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.067 | 0.622 |
| Number of reflections | 37544 | 1911 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 92.0 | 94.3 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 50mM Na HEPES, PEG 1500 |
