5YL7

Proteases from Pseudoalteromonas arctica PAMC 21717 (Pro21717)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	200
Detector technology	CCD
Collection date	2014-05-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	47.948, 74.562, 83.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.400
R-factor	0.13128
Rwork	0.130
R-free	0.15529
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.028
RMSD bond angle	2.371
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.400
Number of reflections	58245
<I/σ(I)>	69
Completeness [%]	98.0
Redundancy	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.16M magnesium chloride, 0.08M Tris-HCl buffer pH 8.5, 24% PEG4000, 20% glycerol