5YKW
Structural basis of the thiol resolving mechanism in yeast mitochondrial 1-Cys peroxiredoxin via glutathione/thioredoxin systems
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97776 |
Spacegroup name | P 32 |
Unit cell lengths | 49.333, 49.333, 50.612 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.650 - 2.080 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.21000 |
RMSD bond length | 0.024 |
RMSD bond angle | 1.757 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.150 |
High resolution limit [Å] | 2.075 | 2.080 |
Number of reflections | 8273 | |
<I/σ(I)> | 7.43 | 2.22 |
Completeness [%] | 99.3 | 92.9 |
Redundancy | 6.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 20% (W/V) PEG-2000 MME ,100mM Tris base/Hydrochloric acid pH 7.0 |