5YKV
The crystal structure of Macrobrachium rosenbergii nodavirus P-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-25 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 125.770, 147.280, 175.915 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.990 - 2.310 |
| R-factor | 0.16029 |
| Rwork | 0.158 |
| R-free | 0.19850 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.447 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.157 | 0.702 |
| Rpim | 0.067 | 0.304 |
| Number of reflections | 71611 | |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 99.3 | 97.6 |
| Redundancy | 5.3 | 4.9 |
| CC(1/2) | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES monohydrate pH 6.5, 1.6 M Magnesium sulfate heptahydrate |
