5YKU
The crystal structure of Macrobrachium rosenbergii nodavirus P-domain with Zn ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 72.874, 72.874, 47.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.870 - 1.390 |
| R-factor | 0.1422 |
| Rwork | 0.142 |
| R-free | 0.15140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.368 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.440 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmeas | 0.075 | 0.356 |
| Rpim | 0.025 | 0.113 |
| Number of reflections | 29388 | |
| <I/σ(I)> | 23.9 | |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 9.6 | 9.9 |
| CC(1/2) | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 10% PEG8000, MES pH 6.5, 0.2 M Zinc acetate |
