5YKN
crystal structure of Arabidopsis thaliana JMJ14 catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.143, 69.221, 100.414 |
| Unit cell angles | 90.00, 104.26, 90.00 |
Refinement procedure
| Resolution | 49.567 - 2.300 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4igq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.851 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.126 | 0.952 |
| Number of reflections | 30445 | 2967 |
| <I/σ(I)> | 16 | 1.4 |
| Completeness [%] | 99.0 | 97.4 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M Na2SO4, 20% PEG 3350, 0.1M bis-tris propane, pH 6.5 |
