5YK9
Crystal structure of selenomethionine-labelled indole prenyltransferase AmbP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-02-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.259, 142.350, 157.147 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.160 - 3.001 |
| R-factor | 0.2401 |
| Rwork | 0.239 |
| R-free | 0.26470 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.160 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.122 | 0.513 |
| Rmeas | 0.556 | |
| Rpim | 0.213 | |
| Number of reflections | 21912 | 2545 |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 7.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG8000, MES, magnesium chloride |
