5YK7
Crystal Structure of Mdm12-Mmm1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 65 |
| Unit cell lengths | 87.560, 87.560, 436.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.142 - 3.799 |
| R-factor | 0.2389 |
| Rwork | 0.238 |
| R-free | 0.26240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gyd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.772 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.870 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.046 | |
| Number of reflections | 18459 | |
| <I/σ(I)> | 27.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 4000, HEPES, Ammonium sulfate |
