5YK5
structure of the human Lamtor4-Lamtor5 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 32 |
| Unit cell lengths | 76.672, 76.672, 52.939 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.030 |
| R-factor | 0.23657 |
| Rwork | 0.233 |
| R-free | 0.29398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.620 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.030 |
| Number of reflections | 22500 |
| <I/σ(I)> | 20.5 |
| Completeness [%] | 100.0 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 0.1M Tris-HCl, pH 8.0, 3.5M sodium formate |
