5YK4
Mismatch Repair Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 89.940, 102.400, 236.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.970 |
| R-factor | 0.247 |
| Rwork | 0.246 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e3m |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.084 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.064 | 0.560 |
| Number of reflections | 43285 | |
| <I/σ(I)> | 8.6 | 2 |
| Completeness [%] | 98.7 | 99.2 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 10-14% (W/V) PEG 4000, 0.1-0.2M MGCL2, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
