5YJD

Structural insights into the CRISPR-Cas-associated ribonuclease activity of Staphylococcus epidermidis Csm3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	I 21 21 21
Unit cell lengths	58.623, 105.760, 155.977
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.880 - 2.260
R-factor	0.19917
Rwork	0.197
R-free	0.24097
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.544
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	52.880
High resolution limit [Å]	2.260
Number of reflections	21671
<I/σ(I)>	18.2
Completeness [%]	98.5
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.16M Calcium acetate, 14% (w/v) PEG8000, 20% (w/v) glycerol, 0.08M Sodium cacodylate pH6.5