5YJC

Structural insights into the CRISPR-Cas-associated ribonuclease activity of Staphylococcus epidermidis Csm6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	61.141, 85.399, 124.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.404 - 2.007
R-factor	0.1805
Rwork	0.178
R-free	0.21840
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.102
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	BUCCANEER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.400
High resolution limit [Å]	2.000
Number of reflections	44031
<I/σ(I)>	31.89
Completeness [%]	99.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M Sodium malonate pH5.0, 12% PEG3350