5YJ7
Structural insight into the beta-GH1 glucosidase BGLN1 from oleaginous microalgae Nannochloropsis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.720, 74.750, 102.940 |
| Unit cell angles | 105.29, 96.10, 90.32 |
Refinement procedure
| Resolution | 27.306 - 1.610 |
| R-factor | 0.1344 |
| Rwork | 0.134 |
| R-free | 0.15150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rgl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.065 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.15) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.040 | 28.040 | 1.640 |
| High resolution limit [Å] | 1.610 | 8.820 | 1.610 |
| Rmerge | 0.034 | 0.018 | 0.258 |
| Rmeas | 0.048 | 0.026 | 0.365 |
| Rpim | 0.034 | 0.018 | 0.258 |
| Total number of observations | 421792 | 2215 | 9964 |
| Number of reflections | 216408 | 1211 | 5230 |
| <I/σ(I)> | 14.5 | 26.7 | 3.4 |
| Completeness [%] | 82.7 | 76.1 | 40.1 |
| Redundancy | 1.9 | 1.8 | 1.9 |
| CC(1/2) | 0.996 | 0.998 | 0.829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 291 | 0.2 M calcium acetate, 0.1 M sodium MES, pH 6.0, and 16% PEG8000 |
