5YIX

Caulobacter crescentus GcrA sigma-interacting domain (SID) in complex with domain 2 of sigma 70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	295.15
Detector technology	PIXEL
Collection date	2016-05-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	65.199, 65.199, 397.934
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.061 - 2.302
R-factor	0.224
Rwork	0.222
R-free	0.25650
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.947
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.110	0.750
Rmeas	0.120	0.780
Rpim	0.030	0.200
Number of reflections	23503	2028
<I/σ(I)>	29.1	2.8
Completeness [%]	98.9	82
Redundancy	20	14.6
CC(1/2)		0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	18% PEG 3350, 0.1M sodium citrate, pH 5.5