5YIU

Caulobacter crescentus GcrA DNA-binding domain (DBD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	30.410, 37.100, 40.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.449 - 1.420
R-factor	0.1846
Rwork	0.183
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gv2
RMSD bond length	0.005
RMSD bond angle	0.908
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.420	1.420
Rmerge	0.080	0.120
Rmeas	0.090	0.130
Rpim	0.040	0.050
Number of reflections	9567	467
<I/σ(I)>	20.9	14.9
Completeness [%]	99.9	99.6
Redundancy	6.2	6.1
CC(1/2)		0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	1.4M sodium citrate, 1M HEPES sodium, pH7.5