5YIN
Hen egg-white lysozyme precipitant-free orthorhombic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.490, 58.040, 67.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.960 - 1.660 |
| R-factor | 0.17484 |
| Rwork | 0.173 |
| R-free | 0.21280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.704 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0171) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.961 | 1.680 |
| High resolution limit [Å] | 1.655 | 1.660 |
| Rmerge | 0.049 | 0.235 |
| Rpim | 0.020 | 0.103 |
| Number of reflections | 14526 | |
| <I/σ(I)> | 25.4 | 6.7 |
| Completeness [%] | 97.8 | 82.2 |
| Redundancy | 7.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | No precipitants, extensive concentration |
