5YIN

Hen egg-white lysozyme precipitant-free orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	30.490, 58.040, 67.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.960 - 1.660
R-factor	0.17484
Rwork	0.173
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.704
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0171)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.961	1.680
High resolution limit [Å]	1.655	1.660
Rmerge	0.049	0.235
Rpim	0.020	0.103
Number of reflections	14526
<I/σ(I)>	25.4	6.7
Completeness [%]	97.8	82.2
Redundancy	7.1	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298	No precipitants, extensive concentration