5YI3
Structure of Lactococcus lactis ZitR, C30S mutant in complex with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.522, 103.058, 104.855 |
Unit cell angles | 90.00, 94.20, 90.00 |
Refinement procedure
Resolution | 42.486 - 2.900 |
R-factor | 0.2078 |
Rwork | 0.205 |
R-free | 0.26250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q5f |
RMSD bond length | 0.009 |
RMSD bond angle | 1.328 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.080 | 0.873 |
Number of reflections | 47584 | 4718 |
<I/σ(I)> | 18 | 1.9 |
Completeness [%] | 98.5 | 98.4 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 293 | 0.1M Sodium cacodylate pH 6.1, 0.05M Magnesium chloride hexahydrate, 0.1M Sodium chloride, 20%(w/v) Polyethylene glycol 4000 |