5YI2
Structure of Lactococcus lactis ZitR, wild type in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.616, 101.568, 101.544 |
| Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
| Resolution | 40.116 - 2.600 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5DMT 3q5f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.244 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.127 | 0.984 |
| Number of reflections | 64593 | 1463 |
| <I/σ(I)> | 11.9 | 2 |
| Completeness [%] | 98.2 | 98.6 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 293 | 0.1M Sodium cacodylate pH 6.1, 0.05M Magnesium chloride hexahydrate, 0.1M Sodium chloride, 20%(w/v) Polyethylene glycol 4000 |
