5YI0
Structure of Lactococcus lactis ZitR, C30AH42A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.038, 86.864, 126.783 |
| Unit cell angles | 90.00, 90.69, 90.00 |
Refinement procedure
| Resolution | 19.549 - 2.300 |
| R-factor | 0.2533 |
| Rwork | 0.251 |
| R-free | 0.30080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yhy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.083 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.076 | 0.747 |
| Number of reflections | 49739 | 2585 |
| <I/σ(I)> | 17.3 | 2.1 |
| Completeness [%] | 97.6 | 99.1 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES pH 7.5, 42%(v/v) Polyethylene glycol 200 |
