5YHX
Structure of Lactococcus lactis ZitR, wild type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.754, 125.793, 153.655 |
| Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
| Resolution | 48.665 - 2.400 |
| R-factor | 0.2237 |
| Rwork | 0.221 |
| R-free | 0.28860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tgn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.081 | 0.769 |
| Number of reflections | 57242 | 2795 |
| <I/σ(I)> | 17.7 | 2 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.05M Magnesium chloride hexahydrate, 0.1M HEPES pH 7.5, 30%(v/v) Polyethylene glycol monomethyl ether 550 |
