5YHV

Crystal structure of an aminotransferase from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-09-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	93.277, 98.224, 198.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.390 - 2.700
R-factor	0.23759
Rwork	0.236
R-free	0.27210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h14
RMSD bond length	0.008
RMSD bond angle	1.326
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.390	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	50648
<I/σ(I)>	12.75
Completeness [%]	99.3
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M Ammonium phosphate, 0.1M Calcium Chloride