5YHU
Crystal structure of the DNA-binding domain of human myelin-gene regulatory factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.122, 105.122, 297.413 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.181 - 1.850 |
| R-factor | 0.2051 |
| Rwork | 0.204 |
| R-free | 0.22750 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.948 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.051 | 0.612 |
| Number of reflections | 54003 | |
| <I/σ(I)> | 28.6 | 1.7 |
| Completeness [%] | 99.4 | 97.6 |
| Redundancy | 4.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 200mM Calcium acetate, 100mM Tris-HCl pH 7.1, 19% PEG 3350 |
